ELISA/assay

Fish Leptin (LEP) ELISA Kit

MBS280395

48-Strip-Wells

440 USD

ELISA Kit

Fish Leptin (LEP) ELISA Kit

[Leptin]

[leptin; Leptin; leptin; leptin; Obese protein; Obesity factor]

[LEP]

[LEP; LEP; OB; OBS; LEPD]

Fish

167

This assay has high sensitivity and excellent specificity for detection of Fish IL-10. No significant cross-reactivity or interference between Fish IL-10 and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 3.12 pg/mL-200 pg/mL. Sensitivity: 1.56 pg/mL (mean of 6 independent assays).

Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: For the quantitative detection of Fish Interleukin 10 (IL-10) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL-10 in Fish serum, plasma. An antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4557715

NP_000221.1

NM_000230.2

P41159

18,641 Da

AMPK Signaling Pathway (198868); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (477129); HIF-1-alpha Transcription Factor Network Pathway (138045); Incretin Synthesis, Secretion, And Inactivation Pathway (187170); Jak-STAT Signaling Pathway (83077)

This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Aug 2017]

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family. Protein type: Cell development/differentiation; Hormone; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q32.1. Cellular Component: cytoplasm; extracellular region; extracellular space. Molecular Function: growth factor activity; hormone activity; peptide hormone receptor binding. Biological Process: adult feeding behavior; angiogenesis; bile acid metabolic process; central nervous system neuron development; cholesterol metabolic process; circadian rhythm; eating behavior; energy reserve metabolic process; fatty acid beta-oxidation; female pregnancy; glucose homeostasis; glucose metabolic process; glycerol biosynthetic process; hormone metabolic process; insulin secretion; interleukin-12 production; intestinal absorption; leptin-mediated signaling pathway; leukocyte tethering or rolling; lipid metabolic process; negative regulation of apoptosis; negative regulation of appetite; negative regulation of autophagy; negative regulation of glucose import; negative regulation of transcription from RNA polymerase II promoter; negative regulation of vasoconstriction; ovulation from ovarian follicle; phagocytosis; placenta development; positive regulation of developmental growth; positive regulation of follicle-stimulating hormone secretion; positive regulation of insulin receptor signaling pathway; positive regulation of ion transport; positive regulation of JAK-STAT cascade; positive regulation of luteinizing hormone secretion; positive regulation of MAPK cascade; positive regulation of myeloid cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling; positive regulation of T cell proliferation; positive regulation of TOR signaling pathway; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of STAT protein; prostaglandin secretion; regulation of angiogenesis; regulation of blood pressure; regulation of bone remodeling; regulation of cell cycle; regulation of endothelial cell proliferation; regulation of gluconeogenesis; regulation of insulin secretion; regulation of intestinal cholesterol absorption; regulation of natural killer cell activation; regulation of natural killer cell mediated cytotoxicity; regulation of natural killer cell proliferation; regulation of nitric-oxide synthase activity; regulation of steroid biosynthetic process; response to activity; response to dietary excess; response to estradiol; response to ethanol; response to hypoxia; response to insulin stimulus; response to vitamin E; sexual reproduction; T cell differentiation; tyrosine phosphorylation of STAT protein. Disease: Leptin Deficiency

48-Strip-Wells

440 USD

96-Strip-Wells

745

5x96-Strip-Wells

3405

10x96-Strip-Wells

6425