ELISA/assay

Human Phosphogluconate dehydrogenase (PGD) ELISA Kit

MBS280393

48-Strip-Wells

440 USD

ELISA Kit

Human Phosphogluconate dehydrogenase (PGD) ELISA Kit

[Phosphogluconate dehydrogenase]

[6-phosphogluconate dehydrogenase, decarboxylating isoform 2; 6-phosphogluconate dehydrogenase, decarboxylating; 6-phosphogluconate dehydrogenase, decarboxylating; phosphogluconate dehydrogenase]

[PGD]

[PGD; PGD; 6PGD; PGDH]

Human

246

This assay has high sensitivity and excellent specificity for detection of Human PGD. No significant cross-reactivity or interference between Human PGD and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PGD in samples. An antibody specific for PGD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGD bound in the initial step. The color development is stopped and the intensity of the color is measured.

751130505

NP_001291380.1

NM_001304451.1

P52209

51,872 Da

Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (343); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Carbohydrates Pathway (1269957); Pentose Phosphate Pathway (198829); Pentose Phosphate Pathway (82928); Pentose Phosphate Pathway (289)

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]

PGD: an oxidative carboxylase that catalyses the decarboxylating reduction of 6-phosphogluconate into ribulose 5-phosphate in the presence of NADP. This reaction is a component of the hexose mono-phosphate shunt and pentose phosphate pathways. Protein type: Carbohydrate Metabolism - pentose phosphate pathway; EC 1.1.1.44; Other Amino Acids Metabolism - glutathione; Oxidoreductase. Chromosomal Location of Human Ortholog: 1p36.22. Cellular Component: cytosol; nucleus. Molecular Function: phosphogluconate dehydrogenase (decarboxylating) activity. Biological Process: D-gluconate metabolic process; pentose biosynthetic process; pentose-phosphate shunt; pentose-phosphate shunt, oxidative branch

48-Strip-Wells

440 USD

96-Strip-Wells

745

5x96-Strip-Wells

3405

10x96-Strip-Wells

6425