ELISA/assay

Plant Calmodulin (CAM) ELISA Kit

MBS280185

48-Strip-Wells

440 USD

ELISA Kit

Plant Calmodulin (CAM) ELISA Kit

[Calmodulin]

[calmodulin; Calmodulin; calmodulin; prepro-calmodulin 1; phosphorylase kinase, delta subunit; calmodulin 1 (phosphorylase kinase, delta)]

[CAM]

[CALM1; CALM1; caM; CAMI; PHKD; CPVT4; DD132; CALML2; CALM; CAM; CAM1; CaM]

CALM_HUMAN

Plant

149

This assay has high sensitivity and excellent specificity for detection of PlantCaM. No significant cross-reactivity or interference between PlantCaM and analogues was observed.

Store at 2-8 degree C.

Samples: Serum, Plasma, Other biological fluids. Assay Type: Sandwich

Sample Volume: 1-200 uL. Precision: Intra-assay Precision (Precision within an assay). Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays). Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Intra-Assay: CV. Inter-Assay: CV. Detection Wavelength: 450 nm

Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM can also make use of the calcium stores in the endoplasmic reticulum, and the sarcoplasmic reticulum. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CaM in samples. An antibody specific for CaM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCaM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CaM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CaM bound in the initial step. The color development is stopped and the intensity of the color is measured.

5542035

AAD45181.1

16,838 Da

Activation Of Ca-permeable Kainate Receptor Pathway (161046); Activation Of CaMK IV Pathway (161042); Activation Of Kainate Receptors Upon Glutamate Binding Pathway (161043); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Adaptive Immune System Pathway (366160); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83097)

This gene encodes a member of the EF-hand calcium-binding protein family. It is one of three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Calmodulin: a calcium-binding small regulatory protein that mediates the control of a large number of enzymes by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Has four functional calcium-binding domains. Phosphorylation and ubiquitination result in a decreased activity. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 14q32.11. Cellular Component: nucleoplasm; spindle pole; centrosome; sarcomere; growth cone; spindle microtubule; cytoplasm; plasma membrane; extracellular region; nucleus; cytosol; vesicle. Molecular Function: protein domain specific binding; nitric-oxide synthase regulator activity; titin binding; calcium ion binding; calcium-dependent protein binding; protein kinase binding; protein serine/threonine kinase activator activity; protein binding; phospholipase binding; N-terminal myristoylation domain binding; thioesterase binding; type 3 metabotropic glutamate receptor binding; phosphoinositide 3-kinase binding; nitric-oxide synthase binding; adenylate cyclase binding. Biological Process: positive regulation of phosphoprotein phosphatase activity; phototransduction, visible light; inositol phosphate metabolic process; nerve growth factor receptor signaling pathway; pathogenesis; signal transduction; nitric oxide metabolic process; positive regulation of protein amino acid dephosphorylation; synaptic transmission; substantia nigra development; platelet degranulation; muscle contraction; positive regulation of cyclic nucleotide metabolic process; response to corticosterone stimulus; epidermal growth factor receptor signaling pathway; platelet activation; mitochondrion organization and biogenesis; fibroblast growth factor receptor signaling pathway; glycogen catabolic process; regulation of heart rate; positive regulation of nitric-oxide synthase activity; organelle organization and biogenesis; response to amphetamine; adenylate cyclase activation; glucose metabolic process; positive regulation of protein amino acid autophosphorylation; regulation of cytokinesis; rhodopsin mediated signaling; G-protein coupled receptor protein signaling pathway; regulation of rhodopsin mediated signaling; phospholipase C activation; carbohydrate metabolic process; innate immune response; positive regulation of cyclic-nucleotide phosphodiesterase activity; regulation of nitric-oxide synthase activity; detection of calcium ion; blood coagulation; response to calcium ion; vascular endothelial growth factor receptor signaling pathway. Disease: Long Qt Syndrome 14; Ventricular Tachycardia, Catecholaminergic Polymorphic, 4

48-Strip-Wells

440 USD

96-Strip-Wells

745

5x96-Strip-Wells

3405

10x96-Strip-Wells

6425