catalog number :
MBS280141
products type :
ELISA Kit
products full name :
Human Tumor necrosis factor alpha (TNF/TNFA/TNFSF2) ELISA Kit
products short name :
[Tumor necrosis factor alpha]
other names :
[tumor necrosis factor alpha; tumor necrosis factor alpha]
other gene names :
[tnfa]
specificity :
This assay has high sensitivity and excellent specificity for detection of Human TIAM1. No significant cross-reactivity or interference between Human TIAM1 and analogues was observed.
storage stability :
Store at 2-8 degree C.
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.31 ng/mL-20 ng/mL. Sensitivity: 0.156 ng/mL (mean of 6 independent assays).
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: For the quantitative detection of Human T-lymphoma invasion and metastasis-inducing protein 1 (TIAM1) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TIAM1 in Human serum, plasma. An antibody specific for TIAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001121579.1
ncbi gb acc num :
NM_001128107.1
size4 :
10x96-Strip-Wells