catalog number :
MBS2800651
products full name :
Rabbit Anti-Human AMPKalpha1/2 (phospho Thr183/172) Polyclonal Antibody
products short name :
[AMPKalpha1/2]
products name syn :
[PRKAa1/2; AMPK; AMPKa1/2; PKA; ACACA kinase; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase]
products gene name :
[AMPKalpha1/2]
reactivity :
Mouse, Rat, Monkey, Pig.
specificity :
Phospho-AMPKalpha1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKalpha1/2 protein only when phosphorylated at T183/172.
purity :
The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.
storage stability :
Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving. Avoid repeated freeze-thaw cycles. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
app notes :
WB=1:500-2000. ELISA=1:10000-40000. IHC=1:100-300. IF=1:50-200. Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
other info1 :
Buffer: PBS (pH 7.4) with 0.5% BSA, 0.02% Proclin 300 and 50% Glycerol. Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189. Predicted MW: 63 kDa
products description :
5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.