ELISA/assay

Human Transmembrane Protein 2 Like Protein (TMEM2L) ELISA Kit

MBS2707307

24-Strip-Wells

230 USD

ELISA Kit

Human Transmembrane Protein 2 Like Protein (TMEM2L) ELISA Kit

[Transmembrane Protein 2 Like Protein (TMEM2L)]

[CEMIP; CCSP1; KIAA1199; Cell Migration Inducing Protein, Hyaluronan Binding]

[transmembrane protein 2-like protein; Transmembrane protein 2-like protein; Transmembrane protein 2-like protein]

[TMEM2L]

Human

1355

This assay has high sensitivity and excellent specificity for detection of TLR9. No significant cross-reactivity or interference between TLR9 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.2-2,000pg/mL. Sensitivity: 12.1pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TLR9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TLR9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TLR9 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TLR9. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TLR9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TLR9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TLR9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

398665106

AFO94029.1

152,601 Da

24-Strip-Wells

230 USD

48-Strip-Wells

385

96-Strip-Wells

515

5x96-Strip-Wells

2150

10x96-Strip-Wells

4000