Multi-species Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit | MyBioSource MBS2707170 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Multi-species Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit

catalog :

MBS2707170

quantity :

24-Strip-Wells

price :

235 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2707170

products type :

ELISA Kit

products full name :

Multi-species Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) ELISA Kit

products short name :

[Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)]

other names :

[Wingless-type MMTV integration site family, member 5A; Protein Wnt-5a; protein Wnt-5a; Wnt family member 5A]

products gene name :

[WNT5A]

other gene names :

[WNT5A; WNT5A; hWNT5A]

reactivity :

Human, Mouse, Rat

sequence length :

365

specificity :

This assay has high sensitivity and excellent specificity for detection of WNT5A. No significant cross-reactivity or interference between WNT5A and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.057ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT5A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT5A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WNT5A in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to WNT5A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WNT5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WNT5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

40352783

ncbi acc num :

AAH64694.1

ncbi mol weight :

40,887 Da

ncbi pathways :

Asymmetric Localization Of PCP Proteins Pathway (941396); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Ca2+ Pathway (941399); Class B/2 (Secretin Family Receptors) Pathway (106378); DNA Damage Response (only ATM Dependent) Pathway (198827); GPCR Ligand Binding Pathway (161020); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Hedgehog Signaling Pathway (83063)

ncbi summary :

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

uniprot summary :

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

395

size3 :

96-Strip-Wells

price3 :

525

size4 :

5x96-Strip-Wells

price4 :

2205

size5 :

10x96-Strip-Wells

price5 :

4105

more info or order :

MyBioSource product webpage