Mouse Interleukin 1 Family, Member 9 (IL1F9) ELISA Kit | MyBioSource MBS2706958 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Interleukin 1 Family, Member 9 (IL1F9) ELISA Kit

catalog :

MBS2706958

quantity :

24-Strip-Wells

price :

235 USD

product information

catalog number :

MBS2706958

products type :

ELISA Kit

products full name :

Mouse Interleukin 1 Family, Member 9 (IL1F9) ELISA Kit

products short name :

[Interleukin 1 Family, Member 9 (IL1F9)]

products name syn :

[IL-1F9; IL36g; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; Interleukin 36, Gamma; Interleukin-1 Homolog 1; Interleukin-1 epsilon; Interleukin 1-Related Protein 2]

other names :

[interleukin 1 family, member 9; Interleukin-36 gamma; interleukin-36 gamma; interleukin 36 gamma; IL-1-related protein 2; IL-1RP2; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 9; IL-1F9; Interleukin-1 homolog 1; IL-1H1]

products gene name :

[IL1F9]

other gene names :

[IL36G; IL36G; IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2; IL1E; IL1F9; IL1H1; IL1RP2; IL-1RP2; IL-1 epsilon; IL-1F9; IL-1H1]

reactivity :

Mouse

sequence length :

169

specificity :

This assay has high sensitivity and excellent specificity for detection of IL1b. No significant cross-reactivity or interference between IL1b and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1,000pg/mL. Sensitivity: 5.9pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL1b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL1b were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1b in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IL1b. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119594019

ncbi acc num :

EAW73613.1

ncbi mol weight :

14,928 Da

ncbi pathways :

Cytokine Signaling In Immune System Pathway (1269310); Immune System Pathway (1269170); Signaling By Interleukins Pathway (1269318)

ncbi summary :

The protein encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

uniprot summary :

Il1f9: Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 2q14.1. Cellular Component: extracellular region; extracellular space. Molecular Function: cytokine activity; interleukin-1 receptor binding. Biological Process: cell-cell signaling; cytokine-mediated signaling pathway; inflammatory response; innate immune response

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

395

size3 :

96-Strip-Wells

price3 :

525

size4 :

5x96-Strip-Wells

price4 :

2205

size5 :

10x96-Strip-Wells

price5 :

4105