Human Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit | MyBioSource MBS2706476 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit

catalog :

MBS2706476

quantity :

24-Strip-Wells

price :

220 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2706476

products type :

ELISA Kit

products full name :

Human Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit

products short name :

[Leucyl/Cystinyl Aminopeptidase (LNPEP)]

products name syn :

[CAP; PLAP; OTASE; IRAP; Oxytocinase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; Placental leucine aminopeptidase]

other names :

[leucyl/cystinyl aminopeptidase; Leucyl-cystinyl aminopeptidase; leucyl-cystinyl aminopeptidase; leucyl and cystinyl aminopeptidase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; IRAP]

products gene name :

[LNPEP]

other gene names :

[LNPEP; LNPEP; CAP; IRAP; PLAP; P-LAP; OTASE; Cystinyl aminopeptidase; IRAP; OTase; P-LAP]

reactivity :

Human

sequence length :

1,006

specificity :

This assay has high sensitivity and excellent specificity for detection of lipopolysaccharide. No significant cross-reactivity or interference between lipopolysaccharide and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.058ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level lipopolysaccharide were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level lipopolysaccharide were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of lipopolysaccharide in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to lipopolysaccharide. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to lipopolysaccharide. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain lipopolysaccharide, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of lipopolysaccharide in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119616490

ncbi acc num :

EAW96084.1

uniprot acc num :

Q9UIQ6

ncbi mol weight :

115,062 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing-Cross Presentation Pathway (477122); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Endosomal/Vacuolar Pathway (477125); Immune System Pathway (106386); Insulin-mediated Glucose Transport Pathway (137981); Membrane Trafficking Pathway (106160); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

ncbi summary :

This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.

size1 :

24-Strip-Wells

price1 :

220 USD

size2 :

48-Strip-Wells

price2 :

355

size3 :

96-Strip-Wells

price3 :

475

size4 :

5x96-Strip-Wells

price4 :

1975

size5 :

10x96-Strip-Wells

price5 :

3675

more info or order :

MyBioSource product webpage