ELISA/assay

Mouse Neuropeptide Y Receptor Y2 (NPY2R) ELISA Kit

MBS2705991

24-Strip-Wells

225 USD

ELISA Kit

Mouse Neuropeptide Y Receptor Y2 (NPY2R) ELISA Kit

[Neuropeptide Y Receptor Y2 (NPY2R)]

[NPY-Y2 receptor]

[neuropeptide Y receptor Y2; Neuropeptide Y receptor type 2; neuropeptide Y receptor type 2; neuropeptide Y receptor Y2; NPY-Y2 receptor; Y2 receptor]

[NPY2R]

[NPY2R; NPY2R; NPY2-R; NPY2-R; Y2 receptor]

Mouse

381

This assay has high sensitivity and excellent specificity for detection of NPY1R. No significant cross-reactivity or interference between NPY1R and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Tissue Homogenates, Cell Lysates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.060ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NPY1R were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NPY1R were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NPY1R in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to NPY1R. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NPY1R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NPY1R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NPY1R in the samples is then determined by comparing the O.D. of the samples to the standard curve.

29824918

AAO92062.1

P49146

42,731 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (i) Signalling Events Pathway (1269576); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); GPCRs, Class A Rhodopsin-like Pathway (198886); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Peptide GPCRs Pathway (198897); Peptide Ligand-binding Receptors Pathway (1269546); Signal Transduction Pathway (1269379)

Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > [Ile-31, Gln-34] PP > [Leu-31, Pro-34] NPY > PP, [Pro-34] PYY and NPY free acid.

24-Strip-Wells

225 USD

48-Strip-Wells

365

96-Strip-Wells

490

5x96-Strip-Wells

2025

10x96-Strip-Wells

3765