Human Claudin 3 (CLDN3) ELISA Kit | MyBioSource MBS2705739 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Claudin 3 (CLDN3) ELISA Kit

catalog :

MBS2705739

quantity :

24-Strip-Wells

price :

220 USD

product information

catalog number :

MBS2705739

products type :

ELISA Kit

products full name :

Human Claudin 3 (CLDN3) ELISA Kit

products short name :

[Claudin 3 (CLDN3)]

products name syn :

[C7orf1; CPE-R2; CPETR2; HRVP1; RVP1; Clostridium Perfringens Enterotoxin Receptor 2; Ventral Prostate.1-Like Protein; CPE-Receptor 2]

other names :

[claudin 3; Claudin-3; claudin-3; claudin 3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; Rat ventral prostate.1 protein homolog; hRVP1]

products gene name :

[CLDN3]

other gene names :

[CLDN3; CLDN3; RVP1; HRVP1; C7orf1; CPE-R2; CPETR2; C7orf1; CPETR2; CPE-R 2; CPE-receptor 2; hRVP1]

reactivity :

Human

sequence length :

220

specificity :

This assay has high sensitivity and excellent specificity for detection of CLCF1. No significant cross-reactivity or interference between CLCF1 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1,000pg/mL. Sensitivity: 6.7pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CLCF1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CLCF1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CLCF1 in mouse serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CLCF1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CLCF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CLCF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CLCF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

2459928

ncbi acc num :

AAC78277.1

uniprot acc num :

O15551

ncbi mol weight :

23,319 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Junction Organization Pathway (160966); Cell-Cell Communication Pathway (477132); Cell-cell Junction Organization Pathway (119532); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Tight Junction Pathway (83071)

ncbi summary :

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]

uniprot summary :

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

size1 :

24-Strip-Wells

price1 :

220 USD

size2 :

48-Strip-Wells

price2 :

355

size3 :

96-Strip-Wells

price3 :

475

size4 :

5x96-Strip-Wells

price4 :

1975

size5 :

10x96-Strip-Wells

price5 :

3675