Human Gasdermin D (GSDMD) ELISA Kit | MyBioSource MBS2705515 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Gasdermin D (GSDMD) ELISA Kit

catalog :

MBS2705515

quantity :

24-Strip-Wells

price :

220 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2705515

products type :

ELISA Kit

products full name :

Human Gasdermin D (GSDMD) ELISA Kit

products short name :

[Gasdermin D (GSDMD)]

products name syn :

[GSDMDC1; DF5L; Gasdermin Domain Containing 1]

other names :

[gasdermin-D; Gasdermin-D; gasdermin-D; gasdermin D; Gasdermin domain-containing protein 1]

products gene name :

[GSDMD]

other gene names :

[GSDMD; GSDMD; DF5L; DFNA5L; FKSG10; GSDMDC1; DFNA5L; GSDMDC1; GSDMD-CT]

reactivity :

Human

sequence length :

484

specificity :

This assay has high sensitivity and excellent specificity for detection of Gasdermin D (GSDMD). No significant cross-reactivity or interference between Gasdermin D (GSDMD) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Tissue Homogenates and Other Biological Fluids. Detection Range: 0.312-20ng/mL. Sensitivity: < 0.122ng/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gasdermin D (GSDMD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gasdermin D (GSDMD) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gasdermin D (GSDMD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gasdermin D (GSDMD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gasdermin D (GSDMD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Gasdermin D (GSDMD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

261878494

ncbi acc num :

NP_001159709.1

ncbi gb acc num :

NM_001166237.1

uniprot acc num :

P57764

ncbi mol weight :

52,801 Da

ncbi summary :

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

uniprot summary :

FKSG10: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]. Protein type: Lipid-binding. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: cytosol; extracellular region; extracellular space; ficolin-1-rich granule lumen; NLRP3 inflammasome complex; nucleoplasm; plasma membrane; tertiary granule lumen. Molecular Function: cardiolipin binding; phosphatidic acid binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-4-phosphate binding; phosphatidylserine binding; protein binding. Biological Process: cellular response to extracellular stimulus; cytolysis; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; inflammatory response; innate immune response; neutrophil degranulation; pore complex assembly; positive regulation of interleukin-1 beta secretion; protein homooligomerization; pyroptosis

size1 :

24-Strip-Wells

price1 :

220 USD

size2 :

48-Strip-Wells

price2 :

355

size3 :

96-Strip-Wells

price3 :

475

size4 :

5x96-Strip-Wells

price4 :

1975

size5 :

10x96-Strip-Wells

price5 :

3675

more info or order :

MyBioSource product webpage