Human Tumor Susceptibility Gene 101 (TSG101) ELISA Kit | MyBioSource MBS2704692 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tumor Susceptibility Gene 101 (TSG101) ELISA Kit

catalog :

MBS2704692

quantity :

24-Strip-Wells

price :

220 USD

product information

catalog number :

MBS2704692

products type :

ELISA Kit

products full name :

Human Tumor Susceptibility Gene 101 (TSG101) ELISA Kit

products short name :

[Tumor Susceptibility Gene 101 (TSG101)]

products name syn :

[TSG10; VPS23; Tumor Susceptibility Gene 10; ESCRT-I complex subunit TSG101]

other names :

[tumor susceptibility gene 101 protein; Tumor susceptibility gene 101 protein; tumor susceptibility gene 101 protein; tumor susceptibility 101; ESCRT-I complex subunit TSG101]

products gene name :

[TSG101]

other gene names :

[TSG101; TSG101; TSG10; VPS23]

reactivity :

Human

sequence length :

285

specificity :

This assay has high sensitivity and excellent specificity for detection of Tumor Susceptibility Gene 101 (TSG101). No significant cross-reactivity or interference between Tumor Susceptibility Gene 101 (TSG101) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids. Detection Range: 0.312-20ng/mL. Sensitivity: < 0.114ng/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Susceptibility Gene 101 (TSG101) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Susceptibility Gene 101 (TSG101) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Susceptibility Gene 101 (TSG101). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Susceptibility Gene 101 (TSG101). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Susceptibility Gene 101 (TSG101), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Tumor Susceptibility Gene 101 (TSG101) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

5454140

ncbi acc num :

NP_006283.1

ncbi gb acc num :

NM_006292.3

uniprot acc num :

Q99816

ncbi mol weight :

31,732 Da

ncbi pathways :

Assembly Of HIV Virion Pathway (106008); Disease Pathway (530764); ESCRT-I Complex Pathway (413473); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Endosomal Sorting Complex Required For Transport (ESCRT) Pathway (205244); Glucocorticoid Receptor Regulatory Network Pathway (138014); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); Internalization Of ErbB1 Pathway (138013)

ncbi summary :

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

uniprot summary :

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426).

size1 :

24-Strip-Wells

price1 :

220 USD

size2 :

48-Strip-Wells

price2 :

355

size3 :

96-Strip-Wells

price3 :

475

size4 :

5x96-Strip-Wells

price4 :

1975

size5 :

10x96-Strip-Wells

price5 :

3675