ELISA/assay

Human Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) ELISA Kit

MBS2703462

24-Strip-Wells

210 USD

ELISA Kit

Human Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) ELISA Kit

[Receptor I For The Fc Region Of Immunoglobulin G (FcgRI)]

[CD64; FCGR1; FCGR1A; FCGR1B; FCGR1C; FcgRIA; CD64A; FcgRIB; CD64B; FcgRIC; CD64C; High affinity immunoglobulin gamma Fc receptor I]

[FcgRI, partial; FcgRI; LOW QUALITY PROTEIN: high affinity immunoglobulin gamma Fc receptor I; FcgRI]

[FcgRI]

[LOC101087936; FcgRI]

Human

296

This assay has high sensitivity and excellent specificity for detection of FcgRI. No significant cross-reactivity or interference between FcgRI and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 78-5,000pg/mL. Sensitivity: 31pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FcgRI were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FcgRI were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FcgRI in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FcgRI. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FcgRI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FcgRI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FcgRI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

577030171

AHH81949.1

33,486 Da

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24-Strip-Wells

210 USD

48-Strip-Wells

345

96-Strip-Wells

460

5x96-Strip-Wells

1890

10x96-Strip-Wells

3510