Human Clusterin (CLU) ELISA Kit | MyBioSource MBS2702985 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Clusterin (CLU) ELISA Kit

catalog :

MBS2702985

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2702985

products type :

ELISA Kit

products full name :

Human Clusterin (CLU) ELISA Kit

products short name :

[Clusterin (CLU)]

products name syn :

[CLU; TRPM2; KUB1; CLI; APOJ; SGP2; AAG4; APO-J; SP-40; Apolipoprotein J; Complement Lysis Inhibitor; 40,Sulfated Glycoprotein 2; Testosterone-Repressed Prostate Message 2]

other names :

[clusterin preproprotein; Clusterin; clusterin; clusterin; Aging-associated gene 4 protein; Apolipoprotein J; Apo-J]

products gene name :

[CLU]

other gene names :

[CLU; CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; APOJ; CLI; KUB1; Apo-J; CLI; TRPM-2]

reactivity :

Human

sequence length :

449

specificity :

This assay has high sensitivity and excellent specificity for detection of CLDN4. No significant cross-reactivity or interference between CLDN4 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Human Tissue Homogenates, Cell Lysates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.055ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CLDN4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CLDN4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CLDN4 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CLDN4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CLDN4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CLDN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CLDN4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

355594753

ncbi acc num :

NP_001822.3

ncbi gb acc num :

NM_001831.3

uniprot acc num :

P10909

ncbi mol weight :

53,643 Da

ncbi pathways :

Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Validated Targets Of C-MYC Transcriptional Repression Pathway (169353)

ncbi summary :

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]

uniprot summary :

CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; Mitochondrial; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8p21.1. Cellular Component: cell surface; cytoplasm; cytosol; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; Golgi apparatus; intracellular; mitochondrial membrane; mitochondrion; nucleus; perinuclear region of cytoplasm; protein complex. Molecular Function: amyloid-beta binding; ATPase activity; chaperone binding; low-density lipoprotein receptor binding; misfolded protein binding; protein binding; protein complex binding; ubiquitin protein ligase binding. Biological Process: antimicrobial humoral response; cell morphogenesis; central nervous system myelin maintenance; chaperone-mediated protein complex assembly; complement activation; complement activation, classical pathway; innate immune response; lipid metabolic process; microglial cell activation; negative regulation of protein homooligomerization; platelet degranulation; positive regulation of NF-kappaB transcription factor activity; positive regulation of nitric oxide biosynthetic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein homooligomerization; positive regulation of receptor-mediated endocytosis; positive regulation of tumor necrosis factor production; protein import; protein stabilization; regulation of complement activation; release of cytochrome c from mitochondria; response to misfolded protein; response to virus; reverse cholesterol transport

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

285

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1475

size5 :

10x96-Strip-Wells

price5 :

2745