ELISA/assay

Human VGF Nerve Growth Factor Inducible (VGF) ELISA Kit

MBS2702971

24-Strip-Wells

210 USD

ELISA Kit

Human VGF Nerve Growth Factor Inducible (VGF) ELISA Kit

[VGF Nerve Growth Factor Inducible (VGF)]

[NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide]

[VGF nerve growth factor inducible; Neurosecretory protein VGF; neurosecretory protein VGF; VGF nerve growth factor inducible]

[VGF]

[VGF; VGF; SCG7; SgVII; NERP-1; NERP-2]

Human

615

This assay has high sensitivity and excellent specificity for detection of VEGFR3. No significant cross-reactivity or interference between VEGFR3 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1,000pg/mL. Sensitivity: 6.4pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VEGFR3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VEGFR3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VEGFR3 in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to VEGFR3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VEGFR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGFR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VEGFR3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

119570586

EAW50201.1

O15240

67,258 Da

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. [provided by RefSeq, Jul 2008]

May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.

24-Strip-Wells

210 USD

48-Strip-Wells

345

96-Strip-Wells

460

5x96-Strip-Wells

1890

10x96-Strip-Wells

3510