Human Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit | MyBioSource MBS2702623 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit

catalog :

MBS2702623

quantity :

24-Strip-Wells

price :

210 USD

product information

catalog number :

MBS2702623

products type :

ELISA Kit

products full name :

Human Phospholipase A2, Lipoprotein Associated (LpPLA2) ELISA Kit

products short name :

[Phospholipase A2, Lipoprotein Associated (LpPLA2)]

products name syn :

[PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2]

other names :

[phospholipase A2, membrane associated; Phospholipase A2, membrane associated; phospholipase A2, membrane associated; phospholipase A2 group IIA; GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A]

products gene name :

[LpPLA2]

other gene names :

[PLA2G2A; PLA2G2A; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2; PLA2B; PLA2L; RASF-A; NPS-PLA2]

reactivity :

Human

sequence length :

144

specificity :

This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Lipoprotein Associated (LpPLA2). No significant cross-reactivity or interference between Phospholipase A2, Lipoprotein Associated (LpPLA2) and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma and Other Biological Fluids. Detection Range: 0.625-40ng/mL. Sensitivity: < 0.286ng/mL

other info2 :

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

products description :

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Lipoprotein Associated (LpPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Phospholipase A2, Lipoprotein Associated (LpPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

239915991

ncbi acc num :

NP_001155201.1

ncbi gb acc num :

NM_001161729.1

uniprot acc num :

P14555

ncbi mol weight :

16,083 Da

ncbi pathways :

Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365)

ncbi summary :

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]

uniprot summary :

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:2925633). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins (PubMed:18635536, PubMed:25398877). Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 (PubMed:18635536, PubMed:25398877). Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:25398877). Induces cell proliferation in an integrin-dependent manner (PubMed:18635536).

size1 :

24-Strip-Wells

price1 :

210 USD

size2 :

48-Strip-Wells

price2 :

330

size3 :

96-Strip-Wells

price3 :

440

size4 :

5x96-Strip-Wells

price4 :

1800

size5 :

10x96-Strip-Wells

price5 :

3345