ELISA/assay

Human Programmed Cell Death Protein 1 Ligand 1 (PDL1) ELISA Kit

MBS2702472

24-Strip-Wells

210 USD

ELISA Kit

Human Programmed Cell Death Protein 1 Ligand 1 (PDL1) ELISA Kit

[Programmed Cell Death Protein 1 Ligand 1 (PDL1)]

[CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1]

[programmed cell death 1 ligand 2; Programmed cell death 1 ligand 2; programmed cell death 1 ligand 2; programmed cell death 1 ligand 2; Butyrophilin B7-DC; B7-DC; CD_antigen: CD273]

[PDL1]

[PDCD1LG2; PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2; B7DC; CD273; PDCD1L2; PDL2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; B7-DC]

Human

273

This assay has high sensitivity and excellent specificity for detection of Programmed Cell Death Protein 1 Ligand 1 (PDL1). No significant cross-reactivity or interference between Programmed Cell Death Protein 1 Ligand 1 (PDL1) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids. Detection Range: 0.156-10ng/mL. Sensitivity: < 0.056ng/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 1 (PDL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 1 (PDL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 Ligand 1 (PDL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 1 Ligand 1 (PDL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 1 Ligand 1 (PDL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Programmed Cell Death Protein 1 Ligand 1 (PDL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

190014605

NP_079515.2

NM_025239.3

Q9BQ51

20,745 Da

Adaptive Immune System Pathway (1269171); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (1269177); Immune System Pathway (1269170); PD-1 Signaling Pathway (1269182)

Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production ().

24-Strip-Wells

210 USD

48-Strip-Wells

330

96-Strip-Wells

440

5x96-Strip-Wells

1800

10x96-Strip-Wells

3345