ELISA/assay

Human Procollagen III N-Terminal Propeptide (PIIINP) ELISA Kit

MBS2702067

24-Strip-Wells

220 USD

ELISA Kit

Human Procollagen III N-Terminal Propeptide (PIIINP) ELISA Kit

[Procollagen III N-Terminal Propeptide (PIIINP)]

[P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide]

[PIIINP]

Human

This assay has high sensitivity and excellent specificity for detection of Procollagen III N-Terminal Propeptide (PIIINP). No significant cross-reactivity or interference between Procollagen III N-Terminal Propeptide (PIIINP) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Double-antibody Sandwich. Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids. Detection Range: 6.25-400ng/mL. Sensitivity: < 2.14ng/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen III N-Terminal Propeptide (PIIINP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen III N-Terminal Propeptide (PIIINP) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen III N-Terminal Propeptide (PIIINP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen III N-Terminal Propeptide (PIIINP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen III N-Terminal Propeptide (PIIINP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Procollagen III N-Terminal Propeptide (PIIINP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

24-Strip-Wells

220 USD

48-Strip-Wells

350

96-Strip-Wells

465

5x96-Strip-Wells

1925

10x96-Strip-Wells

3580