Human Collagen Type II (COL2) ELISA Kit | MyBioSource MBS2702063 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Collagen Type II (COL2) ELISA Kit

catalog :

MBS2702063

quantity :

24-Strip-Wells

price :

210 USD

product information

catalog number :

MBS2702063

products type :

ELISA Kit

products full name :

Human Collagen Type II (COL2) ELISA Kit

products short name :

[Collagen Type II (COL2)]

other names :

[Collagen, type II, alpha 1; Collagen alpha-1(II) chain; collagen alpha-1(II) chain; collagen type II alpha 1 chain; Alpha-1 type II collagenCurated]

products gene name :

[COL2]

other gene names :

[COL2A1; COL2A1; AOM; ANFH; SEDC; STL1; COL11A3]

reactivity :

Human

sequence length :

1487

specificity :

This assay has high sensitivity and excellent specificity for detection of COL2. No significant cross-reactivity or interference between COL2 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 93.75-6,000pg/mL. Sensitivity: <34.82pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL2 in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

95132445

ncbi acc num :

AAI16450.1

ncbi mol weight :

29,781 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795)

ncbi summary :

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

size1 :

24-Strip-Wells

price1 :

210 USD

size2 :

48-Strip-Wells

price2 :

330

size3 :

96-Strip-Wells

price3 :

440

size4 :

5x96-Strip-Wells

price4 :

1800

size5 :

10x96-Strip-Wells

price5 :

3345