Mouse Collagen Type I (COL1) ELISA Kit | MyBioSource MBS2702061 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Collagen Type I (COL1) ELISA Kit

catalog :

MBS2702061

quantity :

24-Strip-Wells

price :

210 USD

product information

catalog number :

MBS2702061

products type :

ELISA Kit

products full name :

Mouse Collagen Type I (COL1) ELISA Kit

products short name :

[Collagen Type I (COL1)]

other names :

[Collagen, type I, alpha 2; Collagen alpha-2(I) chain; collagen alpha-2(I) chain; collagen type I alpha 2 chain; Alpha-2 type I collagen]

products gene name :

[COL1]

other gene names :

[COL1A2; COL1A2; OI4; EDSCV; EDSARTH2]

reactivity :

Mouse

sequence length :

1366

specificity :

This assay has high sensitivity and excellent specificity for detection of COL1. No significant cross-reactivity or interference between COL1 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.2-2,000pg/mL. Sensitivity: 12.3pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

45708783

ncbi acc num :

AAH42586.1

ncbi mol weight :

129,314 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); C-MYB Transcription Factor Network Pathway (138073); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Endothelins Pathway (137958)

ncbi summary :

This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

uniprot summary :

Type I collagen is a member of group I collagen (fibrillar forming collagen).

size1 :

24-Strip-Wells

price1 :

210 USD

size2 :

48-Strip-Wells

price2 :

340

size3 :

96-Strip-Wells

price3 :

450

size4 :

5x96-Strip-Wells

price4 :

1845

size5 :

10x96-Strip-Wells

price5 :

3430