ELISA/assay

Rat Interleukin 1 Beta (IL1b) ELISA Kit

MBS2702038

24-Strip-Wells

185 USD

ELISA Kit

Rat Interleukin 1 Beta (IL1b) ELISA Kit

[Interleukin 1 Beta (IL1b)]

[IL1-B; IL1-Beta; IL1F2; IL-1beta; Interleukin-1 Family Member 2; Catabolin]

[interleukin 1 beta S homeolog; interleukin 1 beta S homeolog; interleukin 1 beta S homeolog]

[IL1b]

[il1b.S; IL-1; il1b; IL-1B; il1b-A]

Rat

283

This assay has high sensitivity and excellent specificity for detection of IL1b. No significant cross-reactivity or interference between IL1b and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rabbit Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 3.12-200pg/mL. Sensitivity: 1.15pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL1b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL1b were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1b in rabbit serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IL1b. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

148226412

NP_001079074.1

NM_001085605.1

Apoptosis Pathway (84655); Apoptosis Pathway (470); Cytokine-cytokine Receptor Interaction Pathway (119282); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125125); Cytosolic DNA-sensing Pathway (124833); Herpes Simplex Infection Pathway (377871); Herpes Simplex Infection Pathway (377865); MAPK Signaling Pathway (84645); MAPK Signaling Pathway (456)

24-Strip-Wells

185 USD

48-Strip-Wells

270

96-Strip-Wells

340

5x96-Strip-Wells

1375

10x96-Strip-Wells

2560