ELISA/assay

Human High Mobility Group Protein 1 (HMG1) ELISA Kit

MBS2701750

24-Strip-Wells

210 USD

ELISA Kit

Human High Mobility Group Protein 1 (HMG1) ELISA Kit

[High Mobility Group Protein 1 (HMG1)]

[HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein]

[high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; high mobility group box 1; High mobility group protein 1; HMG-1]

[HMG1]

[HMGB1; HMGB1; HMG1; HMG3; HMG-1; SBP-1; HMG1; HMG-1]

Human

215

This assay has high sensitivity and excellent specificity for detection of HK2. No significant cross-reactivity or interference between HK2 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.6-1,000pg/mL. Sensitivity: 6.0pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HK2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HK2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HK2 in rat serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to HK2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to HK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4504425

NP_002119.1

NM_002128.5

P09429

24,894 Da

Activated TLR4 Signalling Pathway (106400); Activation Of DNA Fragmentation Factor Pathway (105683); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Apoptosis Pathway (105648); Apoptosis Induced DNA Fragmentation Pathway (105682); Apoptotic Execution Phase Pathway (105677); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DAI Mediated Induction Of Type I IFNs Pathway (576256)

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury (PubMed:27362237). Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogdenic activity (). May be involved in platelet activation (). Binds to phosphatidylserine and phosphatidylethanolamide (). Bound to RAGE mediates signaling for neuronal outgrowth (). May play a role in accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP (PubMed:23303669, PubMed:25549101).

24-Strip-Wells

210 USD

48-Strip-Wells

330

96-Strip-Wells

440

5x96-Strip-Wells

1800

10x96-Strip-Wells

3345