This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Cattle Tumor Necrosis Factor Alpha (TNFa) ELISA Kit
catalog :
MBS2701332
quantity :
24-Strip-Wells
price :
220 USD
product information
catalog number :
MBS2701332
products type :
ELISA Kit
products full name :
Cattle Tumor Necrosis Factor Alpha (TNFa) ELISA Kit
products short name :
[Tumor Necrosis Factor Alpha (TNFa)]
products name syn :
[DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2]
other names :
[tumor necrosis factor alpha; tumor necrosis factor alpha]
products gene name :
[TNFa]
other gene names :
[tnfa]
reactivity :
Bovine
sequence length :
312
specificity :
This assay has high sensitivity and excellent specificity for detection of TNFa. No significant cross-reactivity or interference between TNFa and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Bovine Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.8-500pg/mL. Sensitivity: 2.8pg/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNFa were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNFa were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFa in bovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TNFa. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNFa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi gi num :
189409065
ncbi acc num :
NP_001121579.1
ncbi gb acc num :
NM_001128107.1
size1 :
24-Strip-Wells
price1 :
220 USD
size2 :
48-Strip-Wells
price2 :
345
size3 :
96-Strip-Wells
price3 :
460
size4 :
5x96-Strip-Wells
price4 :
1910
size5 :
10x96-Strip-Wells
price5 :
3545
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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