Human Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit | MyBioSource MBS2701262 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit

catalog :

MBS2701262

quantity :

24-Strip-Wells

price :

210 USD

product information

catalog number :

MBS2701262

products type :

ELISA Kit

products full name :

Human Superoxide Dismutase 3, Extracellular (SOD3) ELISA Kit

products short name :

[Superoxide Dismutase 3, Extracellular (SOD3)]

products name syn :

[SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn]

other names :

[superoxide dismutase 3, extracellular; Extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]; superoxide dismutase 3]

products gene name :

[SOD3]

other gene names :

[SOD3; SOD3; EC-SOD; EC-SOD]

reactivity :

Human

sequence length :

300

specificity :

This assay has high sensitivity and excellent specificity for detection of SOD. No significant cross-reactivity or interference between SOD and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.073ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SOD were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SOD were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SOD in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to SOD. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SOD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SOD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

119613229

ncbi acc num :

EAW92823.1

uniprot acc num :

P08294

ncbi mol weight :

25,851 Da

ncbi pathways :

Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (941400); Folate Metabolism Pathway (198833); Oxidative Stress Pathway (198916); Selenium Pathway (198825); Vitamin B12 Metabolism Pathway (920974); Superoxide Radicals Degradation Pathway (142440)

ncbi summary :

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the conversion of superoxide radicals into hydrogen peroxide and oxygen, which may protect the brain, lungs, and other tissues from oxidative stress. Proteolytic processing of the encoded protein results in the formation of two distinct homotetramers that differ in their ability to interact with the extracellular matrix (ECM). Homotetramers consisting of the intact protein, or type C subunit, exhibit high affinity for heparin and are anchored to the ECM. Homotetramers consisting of a proteolytically cleaved form of the protein, or type A subunit, exhibit low affinity for heparin and do not interact with the ECM. A mutation in this gene may be associated with increased heart disease risk. [provided by RefSeq, Oct 2015]

uniprot summary :

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

size1 :

24-Strip-Wells

price1 :

210 USD

size2 :

48-Strip-Wells

price2 :

330

size3 :

96-Strip-Wells

price3 :

440

size4 :

5x96-Strip-Wells

price4 :

1800

size5 :

10x96-Strip-Wells

price5 :

3345