ELISA/assay

Rat Nerve Growth Factor (NGF) ELISA Kit

MBS2701224

24-Strip-Wells

205 USD

ELISA Kit

Rat Nerve Growth Factor (NGF) ELISA Kit

[Nerve Growth Factor (NGF)]

[NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor]

[nerve growth factor; Nerve growth factor]

[NGF]

[ngf; ngfb; NGF; zNGF]

Rat

194

This assay has high sensitivity and excellent specificity for detection of NGAL. No significant cross-reactivity or interference between NGAL and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rabbit Serum, Plasma, Urine, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.64ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NGAL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NGAL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NGAL in rabbit serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to NGAL. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NGAL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NGAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NGAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

42409517

NP_954680.1

NM_199210.1

Q6YBR5

21,903 Da

ARMS-mediated Activation Pathway (823191); Activation Of TRKA Receptors Pathway (823182); Apoptosis Pathway (83637); Apoptosis Pathway (470); Axonal Growth Stimulation Pathway (823178); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (823169); Ceramide Signalling Pathway (823176); Frs2-mediated Activation Pathway (823190); MAPK Signaling Pathway (83627); MAPK Signaling Pathway (456)

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons ().

24-Strip-Wells

205 USD

48-Strip-Wells

325

96-Strip-Wells

425

5x96-Strip-Wells

1745

10x96-Strip-Wells

3240