Rat Matrix Metalloproteinase 3 (MMP3) ELISA Kit | MyBioSource MBS2701197 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Matrix Metalloproteinase 3 (MMP3) ELISA Kit

catalog :

MBS2701197

quantity :

24-Strip-Wells

price :

220 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2701197

products type :

ELISA Kit

products full name :

Rat Matrix Metalloproteinase 3 (MMP3) ELISA Kit

products short name :

[Matrix Metalloproteinase 3 (MMP3)]

products name syn :

[SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1]

other names :

[matrix metalloproteinase 3, partial; Stromelysin-1; stromelysin-1; matrix metallopeptidase 3; Matrix metalloproteinase-3; MMP-3; Transin-1]

products gene name :

[MMP3]

other gene names :

[MMP3; MMP3; SL-1; STMY; STR1; CHDS6; MMP-3; STMY1; STMY1; SL-1; MMP-3]

reactivity :

Rat

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of MMP2. No significant cross-reactivity or interference between MMP2 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: 0.058ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP2 in mouse serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MMP2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MMP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

90287774

ncbi acc num :

ABD92797.1

uniprot acc num :

P08254

ncbi mol weight :

53,977 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Formation Pathway (645288); Degradation Of Collagen Pathway (730309); Degradation Of Collagen Pathway (827690); Degradation Of The Extracellular Matrix Pathway (576263); Degradation Of The Extracellular Matrix Pathway (827688); EGFR Transactivation By Gastrin Pathway (645296); Extracellular Matrix Organization Pathway (576262); Extracellular Matrix Organization Pathway (827676)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

uniprot summary :

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

size1 :

24-Strip-Wells

price1 :

220 USD

size2 :

48-Strip-Wells

price2 :

350

size3 :

96-Strip-Wells

price3 :

470

size4 :

5x96-Strip-Wells

price4 :

1940

size5 :

10x96-Strip-Wells

price5 :

3605

more info or order :

MyBioSource product webpage