catalog number :
MBS2700741
products type :
ELISA Kit
products full name :
Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit
products short name :
[Brain Derived Neurotrophic Factor (BDNF)]
products name syn :
[Neurotrophin; Abrineurin]
other names :
[brain derived neurotrophic factor; Brain-derived neurotrophic factor; brain-derived neurotrophic factor; brain derived neurotrophic factor]
products gene name :
[BDNF]
other gene names :
[BDNF; BDNF; BDNF]
specificity :
This assay has high sensitivity and excellent specificity for detection of AXL. No significant cross-reactivity or interference between AXL and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.312-20ng/mL. Sensitivity: 0.115ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AXL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AXL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AXL in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to AXL. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to AXL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AXL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AXL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AAZ31074.1
ncbi mol weight :
27,715 Da
ncbi pathways :
MAPK Signaling Pathway (199082); MAPK Signaling Pathway (83956); MAPK Signaling Pathway (456)
uniprot summary :
Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it.
size5 :
10x96-Strip-Wells