Rat Connective Tissue Growth Factor (CTGF) ELISA Kit | MyBioSource MBS2700732 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

catalog :

MBS2700732

quantity :

24-Strip-Wells

price :

220 USD

product information

catalog number :

MBS2700732

products type :

ELISA Kit

products full name :

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

products short name :

[Connective Tissue Growth Factor (CTGF)]

products name syn :

[CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2]

other names :

[connective tissue growth factor; Connective tissue growth factor; connective tissue growth factor; connective tissue growth factor; CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Insulin-like growth factor-binding protein 8; IBP-8; IGF-binding protein 8; IGFBP-8]

products gene name :

[CTGF]

other gene names :

[CTGF; CTGF; CCN2; NOV2; HCS24; IGFBP8; CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8]

reactivity :

Rat

sequence length :

349

specificity :

This assay has high sensitivity and excellent specificity for detection of CST3. No significant cross-reactivity or interference between CST3 and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Rabbit Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50ng/mL. Sensitivity: 0.30ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CST3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CST3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CST3 in rabbit serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CST3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CST3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CST3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CST3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

180924

ncbi acc num :

AAA91279.1

uniprot acc num :

P29279

ncbi mol weight :

35,205 Da

ncbi pathways :

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPARA Activates Gene Expression Pathway (530749); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544); TGF-beta Receptor Signaling Pathway (138024)

ncbi summary :

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

uniprot summary :

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.

size1 :

24-Strip-Wells

price1 :

220 USD

size2 :

48-Strip-Wells

price2 :

350

size3 :

96-Strip-Wells

price3 :

470

size4 :

5x96-Strip-Wells

price4 :

1940

size5 :

10x96-Strip-Wells

price5 :

3605