ELISA/assay

Mouse Fibroblast Growth Factor 15 (FGF15) ELISA Kit

MBS2700661

24-Strip-Wells

225 USD

ELISA Kit

Mouse Fibroblast Growth Factor 15 (FGF15) ELISA Kit

[Fibroblast Growth Factor 15 (FGF15)]

[FGF15]

Mouse

This assay has high sensitivity and excellent specificity for detection of FGF15.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 6.25-100pg/mL. Sensitivity: 2.85pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF15 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF15 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FGF15 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. FGF15 is the FGF-19 ortholog in mice and they have the same amino acid sequence. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to FGF15 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FGF15 and unlabeled FGF15 (Standards or samples) with the pre-coated antibody specific to FGF15. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FGF15 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FGF15 in the sample.

24-Strip-Wells

225 USD

48-Strip-Wells

365

96-Strip-Wells

490

5x96-Strip-Wells

2025

10x96-Strip-Wells

3765