ELISA/assay

General Bilirubin (Bb) ELISA Kit

MBS2700655

24-Strip-Wells

235 USD

ELISA Kit

General Bilirubin (Bb) ELISA Kit

[Bilirubin (Bb)]

[Hematoidin]

[bilirubin UDP-glucuronosyltransferase, bilirubin transferase, HUG-Br1]

[Bb]

General

19

This assay has high sensitivity and excellent specificity for detection of AOPP. No significant cross-reactivity or interference between AOPP and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 308.6-25,000ng/mL. Sensitivity: 104.3ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AOPP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AOPP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AOPP in mouse serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to AOPP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled AOPP and unlabeled AOPP (Standards or samples) with the pre-coated antibody specific to AOPP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of AOPP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of AOPP in the sample.

435833

AAB28635.1

24-Strip-Wells

235 USD

48-Strip-Wells

405

96-Strip-Wells

540

5x96-Strip-Wells

2260

10x96-Strip-Wells

4210