ELISA/assay

General Sphingosine-1-Phosphate (S1P) ELISA Kit

MBS2700637

24-Strip-Wells

255 USD

ELISA Kit

General Sphingosine-1-Phosphate (S1P) ELISA Kit

[Sphingosine-1-Phosphate (S1P)]

[S1P]

General

This assay has high sensitivity and excellent specificity for detection of rT3. No significant cross-reactivity or interference between rT3 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 74.07-6,000pg/mL. Sensitivity: 27.55pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level rT3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level rT3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of rT3 in serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to rT3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled rT3 and unlabeled rT3 (Standards or samples) with the pre-coated antibody specific to rT3. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of rT3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of rT3 in the sample.

24-Strip-Wells

255 USD

48-Strip-Wells

455

96-Strip-Wells

610

5x96-Strip-Wells

2570

10x96-Strip-Wells

4790