ELISA/assay

General Triglyceride (TG) ELISA Kit

MBS2700533

24-Strip-Wells

235 USD

ELISA Kit

General Triglyceride (TG) ELISA Kit

[Triglyceride (TG)]

[TAG; Triacylglycerol; Triacylglyceride]

[triglyceride lipase; Hepatic triacylglycerol lipase; hepatic triacylglycerol lipase; lipase C, hepatic type; Lipase member C]

[TG]

[LIPC; LIPC; HL; HTGL; LIPH; HDLCQ12; HTGL; HL; Hepatic lipase]

General

499

This assay has high sensitivity and excellent specificity for detection of trastuzumab. No significant cross-reactivity or interference between trastuzumab and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Biological Agents. Assay Type: Quantitative Competitive. Detection Range: 2.47-200ng/mL. Sensitivity: 0.94ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level trastuzumab were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level trastuzumab were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of trastuzumab in biological agents. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to trastuzumab has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled trastuzumab and unlabeled trastuzumab (Standards or samples) with the pre-coated antibody specific to trastuzumab. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of trastuzumab in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of trastuzumab in the sample.

339595

AAA61165.1

55,914 Da

LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]

Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.

24-Strip-Wells

235 USD

48-Strip-Wells

400

96-Strip-Wells

535

5x96-Strip-Wells

2245

10x96-Strip-Wells

4175