ELISA/assay

Human Fetal Hemoglobin (HBF) ELISA Kit

MBS2700434

24-Strip-Wells

210 USD

ELISA Kit

Human Fetal Hemoglobin (HBF) ELISA Kit

[Fetal Hemoglobin (HBF)]

[hemoglobin F; Foetal Haemoglobin]

[HBF]

Human

This assay has high sensitivity and excellent specificity for detection of HBb. No significant cross-reactivity or interference between HBb and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma And Erythrocyte Lysates. Assay Type: Quantitative Competitive. Detection Range: 6.17-500ug/mL. Sensitivity: 2.91ug/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HBb were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HBb were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of HBb in human serum, plasma and erythrocyte lysates. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to HBb has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled HBb and unlabeled HBb (Standards or samples) with the pre-coated antibody specific to HBb. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of HBb in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of HBb in the sample.

24-Strip-Wells

210 USD

48-Strip-Wells

330

96-Strip-Wells

440

5x96-Strip-Wells

1800

10x96-Strip-Wells

3345