ELISA/assay

General Noradrenaline (NE) ELISA Kit

MBS2700394

24-Strip-Wells

235 USD

ELISA Kit

General Noradrenaline (NE) ELISA Kit

[Noradrenaline (NE)]

[NA; NAd; Norepinephrine; Norepi]

[noradrenaline transporter; Sodium-dependent noradrenaline transporter; sodium-dependent noradrenaline transporter; solute carrier family 6 member 2; Norepinephrine transporter; NET; Solute carrier family 6 member 2]

[NE]

[SLC6A2; SLC6A2; NET; NAT1; NET1; SLC6A5; NAT1; NET1; SLC6A5; NET]

General

617

This assay has high sensitivity and excellent specificity for detection of NMU. No significant cross-reactivity or interference between NMU and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 16-10,000pg/mL. Sensitivity: 8pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NMU were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NMU were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NMU in mouse serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to NMU has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NMU and unlabeled NMU (Standards or samples) with the pre-coated antibody specific to NMU. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NMU in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NMU in the sample.

189258

AAA59943.1

P23975

57,370 Da

Monoamine Transport Pathway (198851); Na+/Cl- Dependent Neurotransmitter Transporters Pathway (161070); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571)

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.

24-Strip-Wells

235 USD

48-Strip-Wells

395

96-Strip-Wells

530

5x96-Strip-Wells

2210

10x96-Strip-Wells

4115