ELISA/assay

Rat Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ELISA Kit

MBS2700260

24-Strip-Wells

220 USD

ELISA Kit

Rat Cross Linked C-Telopeptide Of Type I Collagen (CTXI) ELISA Kit

[Cross Linked C-Telopeptide Of Type I Collagen (CTXI)]

[CTX-I; CTX1;]

[CTXI]

Rat

This assay has high sensitivity and excellent specificity for detection of Cross Linked C-Telopeptide Of Type I Collagen (CTXI). No significant cross-reactivity or interference between Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Competitive Inhibition. Samples: Serum, Plasma and Other Biological Fluids. Detection Range: 123.5-10000pg/mL. Sensitivity: < 48.1pg/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type I Collagen (CTXI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type I Collagen (CTXI) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cross Linked C-Telopeptide Of Type I Collagen (CTXI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and unlabeled Cross Linked C-Telopeptide Of Type I Collagen (CTXI) (Standards or samples) with the pre-coated antibody specific to Cross Linked C-Telopeptide Of Type I Collagen (CTXI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) in the sample.

24-Strip-Wells

220 USD

48-Strip-Wells

350

96-Strip-Wells

470

5x96-Strip-Wells

1940

10x96-Strip-Wells

3605