General 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit | MyBioSource MBS2700158 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

General 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

catalog :

MBS2700158

quantity :

24-Strip-Wells

price :

235 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2700158

products type :

ELISA Kit

products full name :

General 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit

products short name :

[1,25-Dihydroxyvitamin D3 (DHVD3)]

products name syn :

[DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3]

other names :

[1,25-dihydroxyvitamin D3 24-hydroxylase; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; cytochrome P450 family 24 subfamily A member 1; Cytochrome P450 24A1; Cytochrome P450-CC24]

products gene name :

[DHVD3]

other gene names :

[CYP24A1; CYP24A1; CP24; HCAI; CYP24; HCINF1; P450-CC24; CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase]

reactivity :

General

sequence length :

513

specificity :

This assay has high sensitivity and excellent specificity for detection of DHT. No significant cross-reactivity or interference between DHT and analogues was observed.

storage stability :

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 31.2-2000pg/mL. Sensitivity: 16.15pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DHT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DHT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of DHT in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to DHT has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled DHT and unlabeled DHT (Standards or samples) with the pre-coated antibody specific to DHT. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of DHT in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of DHT in the sample.

ncbi gi num :

306704

ncbi acc num :

AAA62379.1

ncbi mol weight :

43,051 Da

ncbi pathways :

Biological Oxidations Pathway (1270189); Cytochrome P450 - Arranged By Substrate Type Pathway (1270191); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Fat-soluble Vitamins Pathway (1339147); Metabolism Of Vitamins And Cofactors Pathway (1270144); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198887); Phase 1 - Functionalization Of Compounds Pathway (1270190)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D3-26,23-lactone as end product.

size1 :

24-Strip-Wells

price1 :

235 USD

size2 :

48-Strip-Wells

price2 :

395

size3 :

96-Strip-Wells

price3 :

530

size4 :

5x96-Strip-Wells

price4 :

2210

size5 :

10x96-Strip-Wells

price5 :

4115

more info or order :

MyBioSource product webpage