catalog number :
MBS2700150
products type :
ELISA Kit
products full name :
General Progesterone (PG) ELISA Kit
products short name :
[Progesterone (PG)]
products name syn :
[P4; Pregn-4-Ene-3,20-Dione]
other names :
[progesterone receptor isoform B; Progesterone receptor; progesterone receptor; progesterone receptor; Nuclear receptor subfamily 3 group C member 3]
products gene name :
[PG]
other gene names :
[PGR; PGR; PR; NR3C3; NR3C3; PR]
specificity :
This assay has high sensitivity and excellent specificity for detection of PLS3. No significant cross-reactivity or interference between PLS3 and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 0.625-40ng/mL. Sensitivity: 0.299ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLS3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLS3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of PLS3 in human serum, plasma, tissue homogenates or other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PLS3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PLS3 and unlabeled PLS3 (Standards or samples) with the pre-coated antibody specific to PLS3. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PLS3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PLS3 in the sample.
ncbi acc num :
NP_000917.3
ncbi gb acc num :
NM_000926.4
ncbi mol weight :
87,747 Da
ncbi pathways :
Cellular Roles Of Anthrax Toxin Pathway (138076); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848); Nuclear Signaling By ERBB4 Pathway (530744); Oocyte Meiosis Pathway (126909); Oocyte Meiosis Pathway (126872); Ovarian Infertility Genes Pathway (198801); Progesterone-mediated Oocyte Maturation Pathway (119304)
ncbi summary :
This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Sep 2015]
uniprot summary :
The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.
size5 :
10x96-Strip-Wells