ELISA/assay

General Thyroxine (T4) ELISA Kit

MBS2700144

24-Strip-Wells

200 USD

ELISA Kit

General Thyroxine (T4) ELISA Kit

[Thyroxine (T4)]

[3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine]

[T4; T4; T4; T4]

[T4]

[THVgp004]

General

525

This assay has high sensitivity and excellent specificity for detection of spermidine. No significant cross-reactivity or interference between spermidine and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 781.25-200,000pg/mL. Sensitivity: 284.74pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level spermidine were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level spermidine were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of spermidine in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to spermidine has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled spermidine and unlabeled spermidine (Standards or samples) with the pre-coated antibody specific to spermidine. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of spermidine in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of spermidine in the sample.

14250971

NP_116333.1

NC_002794.1

Q91TV9

59,986 Da

24-Strip-Wells

200 USD

48-Strip-Wells

305

96-Strip-Wells

390

5x96-Strip-Wells

1590

10x96-Strip-Wells

2950