ELISA/assay

Mouse total cholesterol (TC) ELISA Kit

MBS269999

48-Strip-Wells

290 USD

ELISA Kit

Mouse total cholesterol (TC) ELISA Kit

total cholesterol (TC)

transcobalamin-2 isoform 2; Transcobalamin-2; transcobalamin-2; macrocytic anemia; vitamin B12-binding protein 2; transcobalamin II; macrocytic anemia; transcobalamin II; Transcobalamin II; TC II; TCII

TC

TCN2; TCN2; II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750; TC2; TC-2; TC II; TCII

TCO2_HUMAN

Mouse

400

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TC concentration. Assay Type: Sandwich. Detection Range: 20 mmol/L-0.312 mmol/L. Sensitivity: 0.06 mmol/L.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse TC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

296080704

NP_001171655.1

NM_001184726.1

44,421 Da

Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway (833804); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009)

This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 22q12.2. Cellular Component: extracellular space; lysosomal lumen; extracellular region; endosome. Molecular Function: metal ion binding; cobalamin binding. Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process. Disease: Transcobalamin Ii Deficiency

48-Strip-Wells

290 USD

96-Strip-Wells

450