ELISA/assay

Rat N methyl d aspartic acid (NMDA) ELISA Kit

MBS269995

48-Strip-Wells

290 USD

ELISA Kit

Rat N methyl d aspartic acid (NMDA) ELISA Kit

N methyl d aspartic acid (NMDA)

neuropilin and tolloid-like protein 1 isoform 3; Neuropilin and tolloid-like protein 1; neuropilin and tolloid-like protein 1; brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1; neuropilin (NRP) and tolloid (TLL)-like 1; Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1

NMDA

NETO1; NETO1; BCTL1; BTCL1; BTCL1

NETO1_HUMAN

Rat

533

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant NMDA concentration. Assay Type: Quantitative Sandwich. Sensitivity: Up to 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat NMDA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

319918840

NP_001188394.1

NM_001201465.1

60,060 Da

This gene encodes a predicted transmembrane protein containing two extracellular CUB domains followed by a low-density lipoprotein class A (LDLa) domain. A similar gene in mice encodes a protein that plays a critical role in spatial learning and memory by regulating the function of synaptic N-methyl-D-aspartic acid receptor complexes in the hippocampus. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

NETO1: a transmembrane protein and component of the N-methyl-D-aspartate receptor (NMDAR) complex. Required to maintain the normal level of NMDAR subunits at synapses. The NMDAR complex is a major excitatory ligand-gated ion channel in the central nervous system and a major mediator of synaptic plasticity. Important in the development and/or maintenance of neuronal circuitry. Neto1-deficient mice have a dramatic decrease in the number of NMDA receptors at synapses, impairing learning and synaptic plasticity. Three isoforms of the human protein are produced by alternative splicing. Isoforms 1 and 2 are retina-specific whereas isoform 3 is found in retina as well as at lower levels in adult and fetal brain. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 18q22.2. Cellular Component: postsynaptic membrane; kainate selective glutamate receptor complex; postsynaptic density; extracellular region; excitatory synapse; cell junction. Molecular Function: ionotropic glutamate receptor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; visual learning; memory

48-Strip-Wells

290 USD

96-Strip-Wells

450