catalog number :
MBS269981
products type :
ELISA Kit
products full name :
Human Rap1 GTPase-GDP dissociation stimulator 1 (RAP1GDS1) ELISA Kit
products short name :
Rap1 GTPase-GDP dissociation stimulator 1 (RAP1GDS1)
other names :
rap1 GTPase-GDP dissociation stimulator 1 isoform 1; Rap1 GTPase-GDP dissociation stimulator 1; rap1 GTPase-GDP dissociation stimulator 1; SMG GDS protein; exchange factor smgGDS; SMG P21 stimulatory GDP/GTP exchange protein; RAP1, GTP-GDP dissociation stimulator 1; Exchange factor smgGDS; SMG GDS protein; SMG P21 stimulatory GDP/GTP exchange protein
products gene name :
RAP1GDS1
other gene names :
RAP1GDS1; RAP1GDS1; GDS1; SmgGDS
uniprot entry name :
GDS1_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PAP1GDS1 concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PAP1GDS1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001093896.1
ncbi gb acc num :
NM_001100426.1
ncbi mol weight :
56,536 Da
ncbi pathways :
Regulation Of RAC1 Activity Pathway (169346)
ncbi summary :
The smg GDP dissociation stimulator (smgGDS) protein is a stimulatory GDP/GTP exchange protein with GTPase activity (Riess et al., 1993 [PubMed 8262526]).[supplied by OMIM, Feb 2010]
uniprot summary :
RAP1GDS1: Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. 3 isoforms of the human protein are produced by alternative splicing. Protein type: GEFs; GEFs, misc. Chromosomal Location of Human Ortholog: 4q23-q25. Cellular Component: mitochondrion; endoplasmic reticulum; cytosol. Molecular Function: GTPase activator activity. Biological Process: reduction of endoplasmic reticulum calcium ion concentration; vascular smooth muscle contraction; myosin filament assembly; elevation of mitochondrial calcium ion concentration