product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Transcription factor IIIB 50 kDa subunit (BRF2) ELISA Kit
catalog :
MBS269976
quantity :
48-Strip-Wells
price :
290 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS269976
products type :
ELISA Kit
products full name :
Mouse Transcription factor IIIB 50 kDa subunit (BRF2) ELISA Kit
products short name :
Transcription factor IIIB 50 kDa subunit (BRF2)
other names :
transcription factor IIIB 50 kDa subunit; Transcription factor IIIB 50 kDa subunit; transcription factor IIIB 50 kDa subunit; BRF-2; hBRFU; hTFIIIB50; B-related factor 2; transcription factor IIB- related factor, TFIIIB50; RNA polymerase III transcription initiation factor BRFU; BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like; BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit; B-related factor 2; BRF-2; hBRFU
products gene name :
BRF2
other gene names :
BRF2; BRF2; BRFU; TFIIIB50; BRFU; PRO1470; TFIIIB50; hTFIIIB50; BRF-2
uniprot entry name :
BRF2_HUMAN
reactivity :
Mouse
sequence length :
419
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant BRF2 concentration. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: Up to 0.06 ng/ml. Assay Type: Sandwich
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse BRF2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi gi num :
22035562
ncbi acc num :
NP_060780.2
ncbi gb acc num :
NM_018310.3
ncbi mol weight :
46,533 Da
ncbi pathways :
Gene Expression Pathway (105937); RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway (160948); RNA Polymerase III Abortive And Retractive Initiation Pathway (160949); RNA Polymerase III Transcription Pathway (106561); RNA Polymerase III Transcription Initiation Pathway (106562); RNA Polymerase III Transcription Initiation From Type 3 Promoter Pathway (106565)
ncbi summary :
This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008]
uniprot summary :
TFIIIB50: General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Belongs to the TFIIB family. Protein type: Transcription regulation. Chromosomal Location of Human Ortholog: 8p11.23. Cellular Component: nucleoplasm. Molecular Function: zinc ion binding. Biological Process: transcription from RNA polymerase III promoter; regulation of transcription, DNA-dependent; transcription initiation; gene expression
size1 :
48-Strip-Wells
price1 :
290 USD
size2 :
96-Strip-Wells
price2 :
450
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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