catalog number :
MBS269974
products type :
ELISA Kit
products full name :
Rabbit Complement 3a (C3a) ELISA Kit
products short name :
Complement 3a (C3a)
other names :
C3a anaphylatoxin chemotactic receptor; C3a anaphylatoxin chemotactic receptor; C3a anaphylatoxin chemotactic receptor; C3a-R; complement component 3 receptor 1; complement component 3a receptor 1
other gene names :
C3AR1; C3AR1; AZ3B; C3AR; HNFAG09; AZ3B; C3R1; HNFAG09; C3AR; C3a-R
uniprot entry name :
C3AR_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Rabbit serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant C3a concentration. Assay Type: Sandwich. Detection Range: 300 ug/ml-4.7 ug/ml. Sensitivity: 0.8 ug/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rabbit C3a monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_004045.1
ncbi gb acc num :
NM_004054.2
ncbi mol weight :
53,864 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)
uniprot summary :
C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; C3a anaphylatoxin receptor activity; complement component C3a receptor activity; phosphoinositide phospholipase C activity. Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; blood circulation; metabolic process; complement receptor mediated signaling pathway; chemotaxis; inflammatory response