product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse E3 ubiquitin-protein ligase RAD18 (RAD18) ELISA Kit
catalog :
MBS269968
quantity :
48-Strip-Wells
price :
290 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS269968
products type :
ELISA Kit
products full name :
Mouse E3 ubiquitin-protein ligase RAD18 (RAD18) ELISA Kit
products short name :
E3 ubiquitin-protein ligase RAD18 (RAD18)
other names :
E3 ubiquitin-protein ligase RAD18; E3 ubiquitin-protein ligase RAD18; E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; RAD18 homolog; RING finger protein 73; RAD18, S. cerevisiae, homolog; postreplication repair protein RAD18; postreplication repair protein hRAD18p; RAD18 E3 ubiquitin protein ligase; Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73
products gene name :
RAD18
other gene names :
RAD18; RAD18; RNF73; RNF73; hHR18; hRAD18
uniprot entry name :
RAD18_HUMAN
reactivity :
Mouse
sequence length :
495
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant RAD18 concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse RAD18 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi gi num :
256818821
ncbi acc num :
NP_064550.3
ncbi gb acc num :
NM_020165.3
ncbi mol weight :
56,223 Da
ncbi summary :
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
uniprot summary :
RAD18: a structural maintenance of chromosomes (SMC) protein involved in postreplication repair of UV-damaged DNA. Has ssDNA binding activity. Interacts with Rad6 through a conserved ring-finger motif. Rad6 is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Protein type: EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase; Ligase; DNA repair, damage; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 3p25.3. Cellular Component: XY body; replication fork; nucleus; chromatin. Molecular Function: Y-form DNA binding; protein binding; zinc ion binding; ubiquitin protein ligase binding; damaged DNA binding; polyubiquitin binding; ligase activity. Biological Process: negative regulation of DNA recombination; protein ubiquitination; spermatogenesis; DNA repair; response to UV
size1 :
48-Strip-Wells
price1 :
290 USD
size2 :
96-Strip-Wells
price2 :
450
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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