ELISA/assay

Porcine Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit

MBS269963

48-Strip-Wells

315 USD

ELISA Kit

Porcine Transcription Factor / Activator Protein 1 (AP-1) ELISA Kit

Transcription Factor / Activator Protein 1 (AP-1)

transcription factor jun-B; Transcription factor jun-B; transcription factor jun-B; activator protein 1; jun B proto-oncogene

AP-1

JUNB; JUNB; AP-1

JUNB_HUMAN

Porcine

347

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Porcine serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AP-1 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine AP-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4504809

NP_002220.1

NM_002229.2

35,879 Da

ATF-2 Transcription Factor Network Pathway (138006); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Corticotropin-releasing Hormone Pathway (920957); Disease Pathway (530764); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); IL6-mediated Signaling Events Pathway (137932); Integrated Pancreatic Cancer Pathway (711360)

JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5 -TGA[CG]TCA-3 . Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily. Protein type: Oncoprotein; Motility/polarity/chemotaxis; Transcription factor. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: nucleoplasm; chromatin. Molecular Function: protein binding; DNA binding; double-stranded DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity. Biological Process: response to drug; response to peptide hormone stimulus; response to light stimulus; transcription initiation from RNA polymerase II promoter; response to cAMP; transcription, DNA-dependent; regulation of cell cycle; embryonic process involved in female pregnancy; decidualization; osteoclast differentiation; trophectodermal cell differentiation; cellular response to hormone stimulus; osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; osteoblast proliferation; response to mechanical stimulus; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; positive regulation of cell differentiation; vasculogenesis; response to corticosterone stimulus

48-Strip-Wells

315 USD

96-Strip-Wells

490