ELISA/assay

Mouse Nicotinamide Adenine Dinucleotide Phosphate Oxidase 2 (NOX2) ELISA Kit

MBS269961

48-Strip-Wells

290 USD

ELISA Kit

Mouse Nicotinamide Adenine Dinucleotide Phosphate Oxidase 2 (NOX2) ELISA Kit

Nicotinamide Adenine Dinucleotide Phosphate Oxidase 2 (NOX2)

NOX2

Mouse

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant NOX2 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse NOX2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

p67phox: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Interacts with SYTL1 and RAC1. Interacts with NCF4. May interact with NOXO1. Belongs to the NCF2/NOXA1 family. Protein type: Oxidoreductase. Chromosomal Location of Human Ortholog: 1q25. Cellular Component: cytoplasm; acrosome; nucleolus; cytosol; NADPH oxidase complex. Molecular Function: protein C-terminus binding; protein binding; electron carrier activity; Rac GTPase binding. Biological Process: respiratory burst; response to drug; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; response to lipopolysaccharide; cellular response to hormone stimulus; antigen processing and presentation of peptide antigen via MHC class I; response to hyperoxia; positive regulation of neuron apoptosis; response to glucose stimulus; cellular defense response; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; response to activity; vascular endothelial growth factor receptor signaling pathway; response to progesterone stimulus; superoxide release; positive regulation of blood pressure; aging. Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Ii

48-Strip-Wells

290 USD

96-Strip-Wells

450