ELISA/assay

Rat Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit

MBS269944

48-Strip-Wells

290 USD

ELISA Kit

Rat Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit

Mothers Against Decapentaplegic Homolog 3 (Smad3)

mothers against decapentaplegic homolog 3 isoform 3; Mothers against decapentaplegic homolog 3; mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3; SMAD family member 3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Smad3

SMAD3; SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3

SMAD3_HUMAN

Rat

381

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Sheep serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant EPI concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Sheep EPI monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substratefor coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

223029442

NP_001138575.1

NM_001145103.1

25,722 Da

AGE/RAGE Pathway (698754); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Adipogenesis Pathway (198832); Alpha6-Beta4 Integrin Signaling Pathway (198807); Androgen Receptor Signaling Pathway (198806); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147809)

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton. Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor. Chromosomal Location of Human Ortholog: 15q22.33. Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; plasma membrane; nuclear inner membrane; nucleus; cytosol; receptor complex. Molecular Function: identical protein binding; ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; beta-catenin binding; protein kinase binding; transcription factor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; phosphatase binding; collagen binding; protein binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor activity; transforming growth factor beta receptor binding. Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; embryonic foregut morphogenesis; positive regulation of stress fiber formation; negative regulation of osteoblast differentiation; negative regulation of mitotic cell cycle; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; regulation of transcription from RNA polymerase II promoter; positive regulation of chondrocyte differentiation; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; negative regulation of apoptosis; endoderm development; evasion of host defenses by virus; wound healing; T cell activation; primary microRNA processing; negative regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of focal adhesion formation; transport; ureteric bud development; positive regulation of transforming growth factor-beta3 production; thyroid gland development; positive regulation of transcription factor import into nucleus; heart looping; caspase activation; intercellular junction assembly and maintenance; transcription initiation from RNA polymerase II promoter; regulation of immune response; regulation of transforming growth factor-beta2 production; regulation of binding; protein stabilization; in utero embryonic development; liver development; positive regulation of bone mineralization; SMAD protein complex assembly; positive regulation of interleukin-1 beta production; immune system development; negative regulation of inflammatory response; response to hypoxia; immune response; gene expression; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration. Disease: Loeys-dietz Syndrome 3

48-Strip-Wells

290 USD

96-Strip-Wells

450