ELISA/assay

Human Phosphorylated Smad 3 (pSmad3) ELISA Kit

MBS269936

48-Strip-Wells

275 USD

ELISA Kit

Human Phosphorylated Smad 3 (pSmad3) ELISA Kit

Phosphorylated Smad 3 (pSmad3)

protein phosphatase 1A; Protein phosphatase 1A; protein phosphatase 1A; IA; PP2C-alpha; protein phosphatase IA; protein phosphatase 2C isoform alpha; protein phosphatase 1A, magnesium dependent, alpha isoform; Protein phosphatase type 1A (formely 2C) Mg-dependent alpha isoform; Protein phosphatase type 1A (formely 2C), Mg-dependent, alpha isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1A; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA

pSmad3

Ppm1a; Ppm1a; Pp2c1; Pp2c1; Pppm1a; PP2C-alpha

PPM1A_RAT

Human

382

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant pSmad3 concentration. Assay Type: Sandwich. Detection Range: 5000 pg/ml-78 pg/ml. Sensitivity: 20 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human pSmad3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

8394012

NP_058734.1

NM_017038.1

42,417 Da

Disease Pathway (1010484); Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway (1010149); Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway (1010072); Gene Expression Pathway (1010398); Generic Transcription Pathway (1010399); IGF1R Signaling Cascade Pathway (1010238); IRS-mediated Signalling Pathway (1010062); IRS-related Events Pathway (1010060); IRS-related Events Triggered By IGF1R Pathway (1010240); Insulin Receptor Signalling Cascade Pathway (1010056)

PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis. Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis. Cellular Component: neuron projection; membrane; voltage-gated calcium channel complex; cytosol; nucleus. Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity; phosphoprotein phosphatase activity. Biological Process: positive regulation of protein export from nucleus; N-terminal protein myristoylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; Wnt receptor signaling pathway; dephosphorylation; positive regulation of transcription, DNA-dependent; negative regulation of NF-kappaB import into nucleus; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transforming growth factor beta receptor signaling pathway; protein amino acid dephosphorylation; positive regulation of Wnt receptor signaling pathway; negative regulation of BMP signaling pathway

48-Strip-Wells

275 USD

96-Strip-Wells

420