catalog number :
MBS269878
products type :
ELISA Kit
products full name :
Human Involucrin (iNV) ELISA Kit
products short name :
Involucrin (iNV)
other names :
involucrin; Involucrin; involucrin; involucrin
other gene names :
IVL; IVL
uniprot entry name :
INVO_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant iNV concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human iNV monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_005538.2
ncbi gb acc num :
NM_005547.2
ncbi mol weight :
68,479 Da
ncbi pathways :
Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway (169349)
ncbi summary :
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]
uniprot summary :
IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: cytoplasm; cornified envelope. Molecular Function: protein binding, bridging; structural molecule activity. Biological Process: keratinocyte differentiation; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; peptide cross-linking; response to UV-B